Fluorescent Corticotropin Releasing Hormone Receptor 2 Internalization Assay Cell Line

Description Green Fluorescent CRF2 Cell Line contains CHO-K1 cells stably expressing CRF2 tagged with tGFP. These Cell lines proliferate in vitro maintaining fluorescent GPCR expression following the cell culture manual. The Green Fluorescent CRF2 Receptor Cell Line allows to assay compounds, or analyze their capability to modulate CRF2 activation and the following redistribution process inside the cells. When a ligand binds to the CRF2 Receptor, it activates a G protein, which internalizes in big and high intensity vesicles. This cell line has been validated measuring Fluorescent CRF2 redistribution by increase of fluorescence surrounding the nuclei using image analysis algorithms. The high reproducibility of this assay allows monitoring CRF2 Receptor redistribution process in High Throughput Screening.