Fluorescent Glucagon-like Peptide-2 Internalization Assay Cell Line

Description Green Fluorescent GLP-2 Receptor Cell Line contains U2OS cells stably expressing GLP2R tagged with tGFP. These Cell lines proliferate in vitro maintaining fluorescent GPCR expression following the cell culture manual. The Green Fluorescent GLP-2 Receptor Cell Line allows to assay compounds, or analyze their capability to modulate GLP2R activation and the following redistribution process inside the cells. When a ligand binds to the GLP-2 Receptor, it activates a G protein, which internalizes in big and high intensity vesicles. This cell line has been validated measuring Fluorescent GLP2R redistribution by increase of fluorescence surrounding the nuclei using image analysis algorithms. The high reproducibility of this assay allows monitoring GLP-2 Receptor redistribution process in High Throughput Screening.