pMEV2HA-MEKK1-K452M

pMEV2HA-MEKK1-K452M is a kinase-dead mutant of the MEKK1 protein, expressed in the pMEV-2HA vector, designed for use in mammalian cells. MEKK1 (Mitogen-Activated Protein Kinase Kinase Kinase 1) is a key regulator in the MAPK signaling pathway, playing a central role in processes such as cellular stress responses, apoptosis, and immune signaling. The K452M mutation introduces a critical alteration to the lysine residue at position 452, rendering MEKK1 kinase-inactive. This mutation effectively blocks MEKK1's ability to phosphorylate downstream effectors, allowing researchers to study kinase-independent functions or use it as a dominant-negative inhibitor in pathway analysis. The HA tag facilitates easy detection and tracking of the protein in cellular assays, making this clone an ideal tool for investigating signal transduction, MAPK pathway regulation, and functional genomics. - 20 ug