Follicle Stimulating Hormone Receptor and CRE-tGFP Stable Co-expressing HEK293 Cell Line

Description Follicle Stimulating Hormone Receptor Cell Line contains HEK293 cells stably expressing human Follicle Stimulating Hormone Receptor with no tag; and the CRE-tGFP reporter gene as a biosensor to monitor cAMP concentration changes. FluoHiTSeeker Cell lines proliferate in vitro maintaining GPCR expression following the cell culture manual. The FSHR Cell Line allows to assay compounds, or analyze their capability to modulate FSHR. When a ligand binds to the FSHR, it activates a G protein, which in turn, triggers a cellular response mediated by cAMP. Due to the presence of the CRE-tGFP reporter gene, this cell line allows to monitor cAMP concentration in living cells measuring fluorescence intensity. This cell line has been validated measuring cAMP concentration changes in the cytosol upon activation with rhFSH. The high reproducibility of this assay allows monitoring FSH receptor activation process in High Throughput Screening.