U2OS Cells stably expressing NK2 Tachykinin Receptor, Calcium Biosensor & cAMP Biosensor

Description U2OS Cells stably expressing NK2 Tachykinin Receptor, Calcium Biosensor & cAMP Biosensor are our multiplex NOMAD (MPX NOMAD) cells. MPX NOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two NOMAD Biosensors to monitor both G-protein pathways. Each vial of MPX NOMAD NK2 Tachykinin Receptor Cell Line contains U2OS cells stably expressing the following constructs: human NK2 Receptor with no tag; Calcium NOMAD Biosensor (Green Fluorescence); and cAMP NOMAD Biosensor (Red Fluorescence). MPX NOMAD NK2 Receptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; both G proteins by Ca2+ & cAMP flux, hence allowing biased activity studies. When an agonist binds to NK2 Receptor two G proteins are activated which, in turn, triggers a cellular response mediated by calcium and cAMP. This leads to an increase of fluorescence intensity of both cAMP and Ca2+ biosensors. So, the activity can be easily quantified on living cells in a dose response manner by fluorescence emission in a microplate reader.